1. Interaction of enterocin CRL35 with model membranes.
Listeria monocytogenes is a bacterium that survives and multiplies at low temperatures, which makes Listeria particularly problematic for food preservation.
Active agents at the level of biological membranes, such as bacteriocins, represent interesting alternatives for the control of L. monocytogenes, which is able to grow in different conditions by manipulating the composition of the membrane fatty acids.
The group studies the interaction of amphipathic peptides with membrane model systems that allows us to find critical molecular determinants of the mechanism of action of enterocin CRL5 in Listeria.
This project is directed by Dr. Fernando Dupuy.
2. Application of enterocin CRL35 in the control of pathogenic enterococci infections.
Enterococcus faecium and Enterococcus faecalis are opportunistic pathogens implicated in a large number of nosocomial infections.
The group studies the interaction of enterocin with clinical isolates belonging to multiresistant enterococcus species.
Our objective is to achieve the use of the bacteriocin enterocin CRL35 as adjuvant therapy, in nosocomial infections.
This project is directed by Dr. Emilse Masias (INSIBIO), in collaboration with Dr. Cecilia Rodríguez and Dr. Lucila Saavedra from CERELA and the Bacteriology Laboratory of the Ernesto C. Padilla Hospital from San Miguel de Tucumán.
3. Design of a culture medium for E. mundtii CRL35
Enterocin CRL35 is secreted in low concentrations by E. mundtii CRL35. This lactic acid bacterium grows in complex culture media; therefore, it is necessary to design a cheaper culture medium that allows production at large scale.
The milk whey is a by-product of the dairy industry that has no major application at present. Therefore, the use of whey as the base for bacterial culture media will give added value to this by-product and will have a positive impact in the region.
The group is developing a whey-based medium as a possible growth substrate for E. mundtii CRL35.
The subject is directed by Dr. Juan Farizano.
4. Inhibition of the enzyme acetylcholinesterase (AChE) by polyphenols.
Acetylcholinesterase is the enzyme responsible for hydrolyzing acetylcholine and is associated with autoimmune, neurodegenerative and infectious pathologies.
Our group studies the inhibition of AChE by phenolic compounds present in white and red grape pomace.
The pomace is a by-product of the wine industry that is obtained from the alcoholic fermentation of the must, and is characterized by its high content of polyphenols.
The theme is directed by Dr. Carlos Minahk.